海洋科学2012，Vol.36Issue(3)：81-88,8.
黄芩苷在中国对虾体内对诺氟沙星消除及细胞色素P450酶的影响
Effects of baicalin on elimination of norfloxacin and activity of CYP450 enzymes in Fenneropenaeus chinensis
摘要
Abstract
The Fenneropenaeus chinensis were divided into three groups fed with different experimental diets. The control group were fed with a non-medicated diet for fifteen days, single treatment group were fed with a non-medicated diet for ten days after a norfloxacin diet for five days, combined treatment group were fed baicalin diet for ten days after a norfloxacin diet for five days. Eight shrimps were randomly selected from the tank and sampled at different days after the first oral administration. Norfloxacin concentrations of hemolymph, hepatopan- creas, gill and muscle were analyzed by HPLC and the activities of CYP1A （ECOD） and CYP2 （APND） was meas- ured in hepatopancreas of F. chinensis by UV spectrophotometry and fluorospectrophotometry. The results showed that the t1/2, was significantly shorter in the combined treatment group than in the single treatment group, and the theoretical withdrawal period of combined treatment group reduced 23.92%, 22.73%, 25.92% and 21.92% com- pared with the single treatment group in hemolymph, hepatopancreas, gill and muscle. The activity of ECOD in the single treatment group was significantly lower than in the control group on the ninth, eleventh （P〈0.01） and thir- teenth days （P〈0.05）, while the activity of ECOD in the combined treatment group was only significantly lower than that in the control group on eleventh day（P〈0.05）, and it was significantly higher than control group on the fifteenth day （P〈0.01）; The activity of APND was significantly lower than the control group on the sixth and sev- enth days in the single treatment group and the combined treatment group （P〈0.05）, while it was significantly higher in the combined treatment group than the control group on the fifteenth day （P〈0.01）. Norfloxacin led to a very significant decrease of the activities of ECOD and APND compared to the control group, and the baicalin as an inducer could stimulate ECOD and APND to increase the enzyme activity in F. chinensis. When the norfloxacin and baicalin were administrated to farmed shrimps as medicated feed to treat bacterial infection, the inhibition of nor- fioxacin and the activation of baicalin could be considered to prevent a drug from affecting other drugs worse for F. chinensis, in order to keep the food safety for the people＇s health and the national economy.关键词
黄芩苷/诺氟沙星/中国对虾（Fenneropenaeus/chinensis）/CYP450Key words
baicalin/norfloxacin/Fenneropenaeus chinensis/CYP450分类
农业科技引用本文复制引用
李健,梁俊平,李小彦,李吉涛,常志强,戴芳钰,赵法箴..黄芩苷在中国对虾体内对诺氟沙星消除及细胞色素P450酶的影响[J].海洋科学,2012,36(3):81-88,8.基金项目
公益性行业（农业）科研专项 （）
农业科技成果转化资金项目 （）
国家虾产业技术体系（nycytx.46）资助 （）
