海洋科学2012，Vol.36Issue(4)：79-85,7.
雨生红球藻铁型超氧化物酶基因的克隆与序列分析
Cloning and sequence analysis of a iron superoxide dismutase from Haematococcus pluvialis
摘要
Abstract
A pair of degenerate primers was designed to amplify specific DNA fragment of FeSOD using cDNA of Haematococcus pluvialis from the homologous sequences. The middle interesting cDNA fragment was obtained by RT-PCR. The full length ofHPFeSOD cDNA was obtained by 5＇RACE and 3＇RACE. The clone contains 1138 bp nucleotides with an open reading frame （ORF） of 684 bp comprising 227 amino acid residues. The sequence shares a high homologue with the following FeSOD： Dunaliella salina, 89%, Chlamydomonas reinhardtii, 83%, Volvox carterif.nagariensis, 78%, Ulva fasciata, 70%, and Cyanobium sp. PCC 7001, 63%. Phylogenetic analysis shows that the FeSOD from H. pluvialis and D. salina are clustered together with genes between fungi and higherplants. The FeSOD of eukaryotic microalgae and higher plants may arise through gene duplication events independently from acommon ancestor.关键词
雨生红球藻（Haematococcus/pluvialis）/超氧化物歧化酶/基因克隆/序列分析Key words
Haematococcus pluvialis/FeSOD/gene cloning/sequence analysis分类
农业科技引用本文复制引用
樊寅卯,张蕾,秦松,严小军..雨生红球藻铁型超氧化物酶基因的克隆与序列分析[J].海洋科学,2012,36(4):79-85,7.基金项目
广东省科技厅农业攻关项目(2010B020308005) （2010B020308005）
广东省海洋与渔业科技推广专项(A200899A05, A200908B05) （A200899A05, A200908B05）
