解放军医学杂志2012，Vol.37Issue(3)：195-199,5.
心房颤动患者循环microRNAs表达谱的初步研究
Preliminary study of circulating microRNA expression profiles in patients with auricular fibrillation
摘要
Abstract
Objective To investigate the expression profiles of the circulating miRNA of patients suffering from persistent and paroxysmal auricular fibrillation (AF) by using microRNA (miRNA) chips. This study will provide the basis for further investigation of the regulatory mechanisms of miRNAs in auricular fibrillation. Methods A total of 5 patients with persistent AF, 5 patients with paroxysmal AF, and 5 healthy volunteers were enrolled for study in the Cardiology Department of the Chinese People's Liberation Army General Hospital. Their whole blood specimens were taken to extract the total RNA serum. MicroRNA chips were used for hybridization, and then miRNA expression profiles were obtained. The volcano plot method was adopted to search for differentially expressed miRNAs, and MEV software -was used to conduct clustering analyses. Results Compared with the healthy control group, 13 miRNA models exhibited significant differential expressions in the serum from persistent and paroxysmal auricular fibrillation patients. Among the 13 models, eight 8 were upregulated, namely, miR-316, miR-3612, miR-634, miR-376a, miR-517b, miR-377*, miR-S90-3p, and miR-664, and five were downregulated, namely, miR-1, kshv-miR-K12-5, miR-378c, miR-204, and miR-27a. A difference in miRNA expression profiles between patients with persistent and paroxysmal AF was discovered. Conclusions The circulating miRNA expression profiles exhibited significant changes, and the study showed that circulating miRNA could be used to study the regulatory mechanisms in the occurrence and development of AF.关键词
心房颤动/微RNAs/芯片分析技术/基因表达谱Key words
atrial fibrillation/ microRNAs/ microchip analytical procedures/ gene expression profile分类
医药卫生引用本文复制引用
张瑜,赵月香,郭红阳,国建萍,林琨,王玉堂,单兆亮..心房颤动患者循环microRNAs表达谱的初步研究[J].解放军医学杂志,2012,37(3):195-199,5.基金项目
国家自然科学基金面上项目(30772886) （30772886）
