解放军医学杂志2012，Vol.37Issue(3)：213-216,4.
LINE-1 ORF-1p对肝脏肿瘤细胞系和肝源永生化细胞系增殖的调控作用
Regulatory effect of LINE-1 ORF-1p on hepatocellular carcinoma cells and proliferation of immortalized hepatocellular cells
摘要
Abstract
Objective The present study investigates the effect of retrotransposon LINE-1 encoding proteins LINE-1 ORF-lp on hepatocellular carcinoma cells and the proliferation ability of immortalized hepatocellular cells. Methods siRNA expression vector for LINE-1 ORF-1 encoding sequence (LINE-1 ORF-lp psilence2.1-U6-siRNA) was structured. Hepatocellular carcinoma cells (Bel-7402, SMMC-7721, HepG2) and immortalized hepatocellular cell (LO2) were transfected. The effect of LINE-1 ORF-lp siRNA expression vector on the expression of LINE-1 ORF-1 encoding protein LINE-1 ORF-lp was detected using the Western blot assay. The effect of the reduction of LINE-1 ORF-lp expression on the proliferation ability of these cells was identified using the MTT method. Result In hepatocellular carcinoma cells (Bel-7402, SMMC-7721, HepG2) and the immortalized hepatocellular cell (LO2), the transfection of LINE-1 ORF-lp siRNA expression vector could reduce the expression level of LINE-1 ORF-lp. Moreover, it could inhibit significantly the proliferation of the above cells on the third day (P<0.05). The inhibition rates are as follows: Bel-7402, 51%; HepG2, 63%; SMMC-7721, 40%; and LO2, 43%. Conclusion The inhibition of LINE-1 ORFlp protein expression can inhibit the proliferation ability of hepatocellular carcinoma cells and the immortalized hepatocellular cell.关键词
肝肿瘤/RNA,小分子干扰/LINE-1 ORF-1p/细胞增殖Key words
liver neoplasms/ RNA, small interfering/ LINE-1 ORF-lp/ cell proliferation分类
医药卫生引用本文复制引用
冯帆,高旭东,陆荫英,张帆,王博,常秀娟,曲建慧,王春平,杨永平..LINE-1 ORF-1p对肝脏肿瘤细胞系和肝源永生化细胞系增殖的调控作用[J].解放军医学杂志,2012,37(3):213-216,4.基金项目
国家重大专项基金(2008ZX10002-018) （2008ZX10002-018）
