解放军医学杂志2012，Vol.37Issue(3)：260-262,3.
葛根素对火药烟雾致支气管上皮细胞损伤的防护效应
Study on the secretion of IL-8 and IL-6 by bronchial epithelial cells (BEAS-2B) injured by black powder smoke and the curative effect of puerarin
摘要
Abstract
Objective The present study investigates the secretion of inflammatory cytokines IL-8 and IL-6 by bronchial epithelial cells (BEAS-2B) injured by black powder smoke and the curative effect of puerarin. Methods BEAS-2B were assigned to pre-treat with puerarin at doses of 100, 50, 25, 12.5|xg/ml respectively, followed by irritation by black powder smoke. MTT method was used to observe the effect of the smoke(irritated for 10 minutes) on the cells. The IL-8 and IL-6 contents in the cell culture supernatant were detected using the ELISA method. Results After the cells were irritated with black powder smoke, their survival rate decreased (48.53% ± 4.44% of control), whereas the content of IL-8 (P<0.05) and IL-6 (P<0.0l) in the cell culture supernatant increased. However, puerarin increased the survival rate of BEAS-2B after irritation and effectively inhibited the secretion of IL-8 and IL-6. Furthermore, the results show that the inhibitory effects were concentration dependent, and had similar statistical differences of P<0.01. Conclusions Black powder smoke can damage BEAS-2B in vitro and induce the secretion of inflammatory cytokines IL-8 and IL-6. However, the Chinese herb medicine puerarin can reverse this injury and reduce the amounts of IL-8 and IL-6 secreted by the injured cells.关键词
烟雾吸入损伤/细胞存活/白细胞介素6/白细胞介素8/葛根素Key words
smoke inhalation injury/ cell survival/ interleukin-6/ interleukin-8/ puerarin分类
医药卫生引用本文复制引用
周莹,兰晓梅,王成彬..葛根素对火药烟雾致支气管上皮细胞损伤的防护效应[J].解放军医学杂志,2012,37(3):260-262,3.基金项目
军队"十一五"计划国际合作项目(06H045) （06H045）
