解放军医学杂志2012，Vol.37Issue(4)：293-296,4.
缺血再灌注损伤时肠道分泌型免疫球蛋白A及其分泌细胞的变化
The alteration in intestinal secretory immunoglobulin A and its secreting cells during ischemia/reperfusion injury
摘要
Abstract
Objective To investigate the change in intestinal secretion immunoglobulin A (slgA) level and IgA-secreting cells during ischemia/reperfusion (I/R) injury. Methods Forty-eight BALB/c mice were randomly divided into 6 experimental groups in accordance with different reperfusion times (R2h, R6h, R12h, R24h, and R72h group), and one sham group (n = 8). Bacterial translocation to distant organs (lung, spleen, and mesenteric lymph nodes) was observed. The slgA level of the intestinal tract was measured by enzyme-linked immunosorbent assay (ELISA). The B cell subgroup in the lymphocytes related to the intestinal tract was measured by flow cytometry. Results The bacterial translocation occurred during I/R injury, and the intestinal slgA level decreased, and they showed an obvious negative correlation (r2 = 0.729). With the increase in intestinal I/R injury, the ratio of IgM+B220+ cells in the gut-associated lymphoid tissue increased, whereas the proportion of IgA+B220+ cells decreased. The most significant change was found in R12h group (P<0.0l). Conclusions The proportion of IgM+ B cells in the gut-associated lymphoid tissue increased, whereas that of IgA+ B cells reduced during I/R injury. These phenomena may cause slgA level to reduce and bacterial translocation of the distant organs to occur.关键词
免疫球蛋白A,分泌/IgA+B细胞/IgM+B细胞/再灌注损伤/细菌移位Key words
immunoglobulin A,secretory/IgA+B cells/IgM+B cells/reperfusion injury/bacterial translocation分类
医药卫生引用本文复制引用
孙立群,李维勤,李秋荣,李宁,黎介寿..缺血再灌注损伤时肠道分泌型免疫球蛋白A及其分泌细胞的变化[J].解放军医学杂志,2012,37(4):293-296,4.基金项目
国家自然科学基金重点项目(30830098) （30830098）
全军"十一五"医药卫生科研基金(06G041) （06G041）
