解放军医学杂志2012，Vol.37Issue(4)：370-374,5.
900MHz电磁辐射对体外培养大鼠海马神经干细胞超微结构的影响
Effects of 900 MHz electromagnetic radiation on ultrastructure of rats′ hippocampal neural stem cells in vitro
摘要
Abstract
Objective To investigate the effect of 900MHz electromagnetic radiation on the ultrastructure of rat hippocampal neural stem cells (NSCs) in vitro in order to provide basic materials for studying the biological effects of electromagnetic wave on the central nervous system. Methods Rat NSCs were divided into sham group, Radii group and Radi2 group, and they were respectively exposed to 900 MHz electromagnetic wave at power density of 0, 1, and 3mW/cm in vitro. Cells in Radii group and Radi2 group were sub-grouped according to the way radiation was given: continuous irradiation, in which cells were exposed on the second day after culture for 2h per day for 6 consecutive days; single exposure to irradiation, in which cells were exposed for 12h on the sixth day after the culture. The ultrastructural changes on the surface of the cells were observed with atomic force microscope (AFM), whereas the ultrastructural changes in the cells were observed with transmission electron microscope (TEM). Results After 900 MHz electromagnetic radiation, when compared with the sham group (OmW/cm2), it was shown that the surface of neural stem cells in the exposure groups (l and 3mW/cm ) became rough, and there were some changes such as "cavitation" and "fissure formation" in the membrane. The intracellular ultrastructure was found to have obviously disrupted in the exposure groups, such as homogenization of cytoplasm, obvious change organelle structure, morphological damage of structure of nucleus, nuclear membrane disappearance, and chromatin pyknosis, and the changes were more obvious in Radi2 group. Compared with the sham group, the surface roughness (Ra) of cells in the exposure group was significantly intensified (P<0.05), and it was higher in Radi2 group than that in Radii group (P<0.05). Conclusion A 900MHz electromagnetic radiation may cause injury changes in NSCs membrane and ultrastructure in vitro, and the extent of injury may be related to the dosage of microwave irradiation.关键词
神经干细胞/海马/微波/显微镜检查,原子力/显微镜检查,电子,透射/细胞膜Key words
neural stem cells/hippocampus/microwaves/microscopy,atomic force/microscopy,electron,transmission/cell membrane分类
医药卫生引用本文复制引用
罗海水,储卫华,万子兵,胡胜利,胡荣,吴南,胡波,冯华,朱刚..900MHz电磁辐射对体外培养大鼠海马神经干细胞超微结构的影响[J].解放军医学杂志,2012,37(4):370-374,5.基金项目
国家自然科学基金面上项目(30770508) （30770508）
