江苏大学学报（医学版）2012，Vol.22Issue(3)：198-202,5.
全反式维甲酸对肝癌HepG2细胞chemerin基因表达的调控
All-trans retinoic acid regulates expression of chemerin in liver HepG2 cell strain
摘要
Abstract
Objective: To investigate the effect and mechanism of all-trans retinoic acid (ATRA) in regulating the expression of chemerin. Methods: HepG2 cells were treated with ATRA in different doses and time. Chemerin mRNA expression level was examined by real-time PCR and Western blotting was used to analyze the expression of chemerin protein. Four consecutive fragments of the chemerin promoter region were individually inserted into reporter plasmid. A luciferase assay was used to study the effect of ATRA on chemerin promoter region. Results; Treating HepG2 cells with ATRA caused dose-dependent increase in the expression of chemerin mRNA and protein. Levels of chemerin reached a maximum level of about two-fold of control at a concentration of 10 μmol/L. Treating HepG2 cells with ATRA also caused time-dependent increase in the expression of chemerin protein, and caused increase in the expression of chemerin mRNA. The expression of chemerin protein increased markedly 36 h after the addition to the cells of ATRA at a concentration of 10 μmol/L. Deletion of the -258bp to + 121 bp region of the chemerin promoter sharply reduced the transactivation efficiency by ATRA- RARβ, suggesting a high possibility that ATRA-RARβ regulation site was within -258 bp/ -90 bp region. Conclusion; ATRA increased the expression of chemerin, and RARβ mediated ATRA-induced chemerin expression.关键词
全反式维甲酸/chemerin/启动子/HepG2细胞Key words
all-trans retinoic acid/chemerin/promoter/ HepG2 cell strain分类
医药卫生引用本文复制引用
邓玉杰,张强,王慧,杨键,张迪,杨颖..全反式维甲酸对肝癌HepG2细胞chemerin基因表达的调控[J].江苏大学学报（医学版）,2012,22(3):198-202,5.基金项目
国家自然科学基金资助项目(30971387) （30971387）
