江苏大学学报（医学版）2012，Vol.22Issue(3)：227-230,4.
辛伐他汀对多柔比星损伤心肌细胞钙稳态作用的研究
Effect of simvastatin on the calcium homeostasis of myocardium cell injury induced by doxorubicin
潘建业 1陈星 1张赢予 1周艳芳 1张国辉1
作者信息
- 1. 江苏大学附属人民医院心内科,江苏镇江212002
- 折叠
摘要
Abstract
Objective: To investigate the protection effect of simvastatin ( SIM) on the myocardium cell injury induced by doxorubiein ( DOX). Methods: The neonatal SD rats cardiomyocytes cultured for 72 hours were divided randomly into five groups; control group,DOX damnified group,DOX +SIM( 1 μmol/L) group,and DOX + S1M( 10 μmol/L)group, and DOX + SIM(20 μmol/L)group. After24 hours further cultivation, the survival ratio of cardiomyocytes were determined by MTT assay; detecting the [ Ca2+ ] I fluorescence intensity by Fluo-3/AM, and Western blot was used to evaluate SERCA2a protein synthesis. Results; Compared to the control group, in the DOX group , the survival ratio of cardiomyocytes were significantly descended( P <0.01); the [ Ca2+ ] I fluorescence intensity was significantly increased( P <0.01), the synthesis of SERCA2a was significantly descended(P < 0. 01). Compared to the DOX group, in the SIM groups, the survival ratio of cardiomyocyte was significantly increased(P <0. 01), the [ Ca 2+ ] I fluorescence intensity was significantly descended(P <0.01) , the synthesis of SERCA2a protein was obviously ameliorated(P<0.01). Conclusion; SIM can protect myocardium cell injury induced by DOX, which may be related to partly by ameliorate the synthesis of SERCA2a and the inhibition of calcium overloading.关键词
辛伐他汀/多柔比星/心肌细胞/钙超载/肌浆网Ca2+-ATP酶Key words
simvastalin/doxorubicin/cardiomyocyte/calcium overloading/ SERCA2a分类
医药卫生引用本文复制引用
潘建业,陈星,张赢予,周艳芳,张国辉..辛伐他汀对多柔比星损伤心肌细胞钙稳态作用的研究[J].江苏大学学报（医学版）,2012,22(3):227-230,4.
