江苏大学学报（医学版）2012，Vol.22Issue(3)：236-241,6.
FA/BRCA途径参与肺癌细胞对顺铂耐药的机制
Effect of FA/BRCA pathway in cisplatin resistance of lung cancer cells
胡一明 1李坚 1陈永昌 2吴燕2
作者信息
- 1. 江苏大学附属医院呼吸科,江苏镇江212001
- 2. 江苏大学基础医学研究所,江苏镇江212013
- 折叠
摘要
Abstract
Objective: To study the effect of cisplatin on expression levels of FANCC,FANCF,FANCL in lung cancers, and explore the effect of FA/BRCA pathway in the mechanism of cisplatin resistance of lung cancer cells. Methods: Inhibition rate of lung cancer cell lines(A549 and Calu-1) after treatment of variant concentration cisplatin was measured using the CCK-8 assay. The expression levels of FANCC, FANCF and FANCL mRNA was detected by real-time fluorogentic quantitative PCR (RFQ-PCR). The expression of FANCC, FANCF, FANCL proteins was determined by Western blotting. Results; The rate of proliferation inhibiting of lung cancer cells is increased in line with the prolong time of cisplatin treatment (P < 0.05 ) and the increase of cisplatin concentration (P < 0.05 ). The inhibitory concentration of 50 percent in Calu-1 cells is significantly higher than in A549 cells. After treatment of 5 - 10 μg/ml and 20μg/ml cisplatin, FANCF and FANCL mRNA expression levels in A549 cells are similar to control group and markedly lower compared with control group, respectively. However, FANCC and FANCF mRNA levels in Calu-1 cells are initiatory increased and then decreased after treatment of variant concentrations cisplatin. The expressions of FANCC, FANCF and FANCL protein in A549 cells were decreased during the treatment of 5 -20 μg/ml cisplatin as compared with control group. In Calu-1 cells, the expression levels of FANCF protein were significantly increased compared with control group during the treatment of 5 -10 μg/ml cisplatin. Conclusion: The cisplatin resistance in Calu-1 cells is significantly increased than in A549 cells. The higher expression of FANCF protein of FA/BRCA pathway may be one of mechanisms for increased resistantto cisplatin in Calu-1 cells.关键词
肺癌细胞/顺铂/FA/BRCA途径/耐药Key words
lung cancer cell/ cisplatin/ Fanconi anemia-BRCA pathway/ drug resistance分类
医药卫生引用本文复制引用
胡一明,李坚,陈永昌,吴燕..FA/BRCA途径参与肺癌细胞对顺铂耐药的机制[J].江苏大学学报（医学版）,2012,22(3):236-241,6.
