抗HCCR单克隆抗体的制备及其鉴定OACSTPCD
Preparation and identification of monoclonal antibody against human cervical cancer oncogene
目的 制备抗人宫颈癌癌基因(HCCR)单克隆抗体(mAb),并进行鉴定.方法 以HCCR重组蛋白免疫BALB/c小鼠,取免疫小鼠的脾细胞和同系小鼠的骨髓瘤细胞SP2/0进行细胞融合,常规筛选杂交瘤细胞.腹水诱导法制备抗人HCCR单克隆抗体,并经蛋白A亲合层析进行纯化.用Westem blot和免疫荧光等方法检测HCCR mAb的特异性.用纯化HCCR蛋白进行包被,建立间接ELISA方法,检测系列稀释的HCCR mAb,确定其灵敏度.结果 筛选出2…查看全部>>
Objective To prepare and identify monoclonal antibody against human cervical cancer oncogence (HCCR). Methods Purified recombinant HCCR protein was used as antigen to immunize BALB/c mice. The isolated spleen cells from the immunized mice were fused with SP2/0. Monoclonal antibodies against HCCR were prepared by normal hybridoma technology. Purified mAb was prepared by protein A chromatography. To identify the characteristics of HCCR mAbs, Western blo…查看全部>>
郝波;林艳;张国新;施瑞华
南京医科大学第一附属医院消化实验室,江苏南京,210029南京医科大学第一附属医院消化实验室,江苏南京,210029南京医科大学第一附属医院消化实验室,江苏南京,210029南京医科大学第一附属医院消化实验室,江苏南京,210029
医药卫生
HCCR单克隆抗体杂交瘤
human cervical cancer oncogence monoclonal antibody hybridoma
《实用临床医药杂志》 2012 (3)
6-9,4
江苏省卫生厅科研项目基金(B52007070)江苏省"六大人才高峰"基金(07-B-017)和江苏省医学重点人才基金(RC2007049)资助
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