江苏大学学报（自然科学版）2012，Vol.33Issue(2)：130-133,186,5.DOI:10.3969/j.issn.1671-7775.2012.02.002
脉冲超声波对黄粉虫幼虫水溶性蛋白结构的影响
Influence of pulse ultrasonic on water soluble protein conformation of Tenebrio molitor( L.) larva
摘要
Abstract
The effects of pulse ultrasonic treatment with power of 1 000 W on water soluble protein conformation of Tenebrio molitor ( L. ) larva were investigated. The results show that the ultraviolet absorp- , tion of protein is increased within 25 min ultrasonic treatment. The surface hydrophobicity has remarkable change with maximum value at 10 min, and decreases gradually from 10 to 25 min. The free sulfhydryl ( SH) content decreases slightly after pulse ultrasonic treatment for 5 min,and then increases gently. Protein particle size decreases with treatment time from 0 to 15 min, and increases significantly after 20 min treatment. The CD analysis indicates that α-helix content is decreased, while β-structure and random coil content are increased by pulse ultrasonic treatment. SDS-PAGE demonstrates that the primary structure of water soluble protein is not changed by pulse ultrasonic treatment.关键词
脉冲/超声波/黄粉虫幼虫/水溶性蛋白/结构Key words
pulse/ ultrasonic/ larva of Tenebrio molitor (L. )/ water soluble protein/ conformation分类
轻工纺织引用本文复制引用
代春华,汤坚,顾小红,马海乐..脉冲超声波对黄粉虫幼虫水溶性蛋白结构的影响[J].江苏大学学报（自然科学版）,2012,33(2):130-133,186,5.基金项目
国家"863"计划项目(2007AA10Z321) （2007AA10Z321）
