军事医学2012，Vol.36Issue(5)：388-391,4.
nMSP-DHPLC检测粪便SEPT9基因甲基化及其在结直肠癌诊断中的应用
Detection of SEPT9 gene methylation by nMSP-DHPLC in stool DNA and its employment in early diagnosis of colorectal cancer
摘要
Abstract
Objective To investigate the value of analyzing septin( SEPT9 ) gene methylation in stool DNA for diagnosis of colorectal cancer. Methods The colorectal carcinoma, para-carcinoma tissues,and stool SEPT9 gene methylation were detected by nested methylation specific PCR( nMSP ) and denatured high performance liquid chromatography ( DHPLC ) in 87 colorectal cancer cases. The sensitivity and specificity of SEPT9 methylation marker for colorectal cancer diagnosis were analyzed. Results Among 87 colorectal cancer cases, SEPT9 gene methylation was found in 80. 5%( 70/87 ) of colorectal carcinoma tissues and 8. 0%( 7/87 ) of para-carcinoma tissues, respectively. The sensitivity and specificity of SEPT9 were 80.5% and 91. 9% , respectively, and the stool DNA was almost as sensitive as cancer tissues in diagnosis of colorectal cancer. The methylation of SEPT9 was significantly associated with elders above 65 years old and the right colon cancer. Conclusion Abnormal methylation of SEPT9 occurs frequently in colorectal cancer, especially in the elders and the right colon cancer, SEPT9 gene mehylation in stool DNA can be used as a candidate tumor marker for early diagnosis of colorectal cancer.关键词
SEPT9/甲基化/粪便/DNA/结直肠肿瘤/色谱法,高压液相/诊断Key words
SEPT9/methylation/ feces/ DNA/ colorectal neoplasms/ chromatography, high pressure liquid/ diagnosis分类
医药卫生引用本文复制引用
赵慧霞,李秋文,董伟伟,段昕妤,朱建华,王如良,郝怡鑫,叶明,肖文华..nMSP-DHPLC检测粪便SEPT9基因甲基化及其在结直肠癌诊断中的应用[J].军事医学,2012,36(5):388-391,4.基金项目
解放军总医院苗圃基金资助(09KMM30) （09KMM30）
