军医进修学院学报2012，Vol.33Issue(3)：248-250,254,4.
抑制LRP16增加Hela细胞对足叶乙甙的敏感性
Inhibition of LRP16 increases sensitivity of Hela cells to etoposide
摘要
Abstract
Objective To study the effect of LRP16 expression on etoposide-induced apoptosis of tumor cells and its possible molecular mechanism. Methods Hela cells were transfected with siRNA, control siRNA, siRNA-374 with an inhibiting rate of 90% for LRP16 expression, and siRNA-668 with an inhibiting rate of 60% for LRP16 expression, and then treated with etoposide to induce DNA damage. Viability of Hela cells was assayed with CCK-8. Effect of inhibiting LRP16 expression on apoptosis of Hela cells was detected by flow cytometry. mRNA level in downstream target genes related with NF- k B was measured by RT-PCR after LRP16 expression was inhibited. Results After treated with etoposide(100|i.mol/L), the inhibited LRP16 expression significantly increased the proliferation and apoptosis of Hela cells in a dose-dependent manner(P<0.05). RT-PCR showed that down-regulation of LRP16 expression decreased the transcription activity of NF- k B and the mRNA level in downstream target genes related with NF- k B. Conclusion LRP16 plays an important role in etoposide-induced apoptosis of Hela cells by down-regulating the activity of NF- k B. Our experiment proves that inhibiting LRP16 expression increases the sensitivity of Hela cells to etoposide.关键词
肿瘤/核转录因子-κB/LRP16基因Key words
neoplasms/ nucler factor- k B/ LRP16 gene分类
医药卫生引用本文复制引用
段玉坤,伍志强,马晓星,赵亚力,韩为东,孟元光..抑制LRP16增加Hela细胞对足叶乙甙的敏感性[J].军医进修学院学报,2012,33(3):248-250,254,4.基金项目
国家自然科学基金项目(81071617,81001184) （81071617,81001184）
