军医进修学院学报2012，Vol.33Issue(6)：649-652,4.
Bcl2-siRNA提高人肺腺癌细胞A549/DDP对顺铂敏感性的研究
Bcl2-siRNA increases sensitivity of human lung adenocarcinoma A549/DDP cells to cisplatin
摘要
Abstract
Objective To study the effect of Bcl2-siRNA on sensitivity of cisplatin-resistant human lung adenocarcinoma A549/DDP cells to cisplatin. Methods Forty-eight hours after Bcl2-siRNA was transfected with cisplatin-resistant human lung adenocarcinoma A549/DDP cells, a fresh cisplatin solution was prepared by adding different concentrations of cisplatin(0 μ g/ml, 3 μ g/ml, 6 μ g/ml, 9μg/ml, 12 μ g/ml, 15 μ g/ml) into A549/DDP cells to measure their growth-inhibition rate by CCK-8 assay. Expression of Bcl-2 mRNA and protein in A549/DDP cells-transfected Bcl2-siRNA was detected by Real time RT-PCR and Western blot, respectively. Results The expression of Bcl-2 mRNA and protein in A549/DDP cells-transfected Bcl2-siRNA decreased 92.6%(P<0.05) while the sensitivity of A549/DDP cells to cisplatin increased 57.8%(P<0.05). Conclusion Bcl2-siRNA can decrease the expression of Bcl-2 mRNA and protein in A549/DDP cells, and increase the sensitivity of A549/DDP cells to cisplatin.关键词
A549/DDP/小干扰RNA/Bcl-2/顺铂/耐药Key words
A549/DDP/ siRNA/ Bcl-2/ cisplatin/ drug resistance分类
医药卫生引用本文复制引用
张学林,余秉翔..Bcl2-siRNA提高人肺腺癌细胞A549/DDP对顺铂敏感性的研究[J].军医进修学院学报,2012,33(6):649-652,4.基金项目
北京市自然科学基金项目(7113165) （7113165）
