军医进修学院学报2012，Vol.33Issue(6)：659-661,671,4.
不同抗凝剂和激活剂组合在自体富血小板血浆凝胶支架促进脂肪干细胞增殖的影响
Effect of combined anticoagulants and agonists on proliferation of adipose derived stromal cells in auto-platelet rich plasma gel stent
张宁 1李放 2罗涛1
作者信息
- 1. 北京军区总医院,骨科,北京,100700
- 2. 解放军总医院/军医进修学院,北京,100853
- 折叠
摘要
Abstract
Objective To study the effect of combined anticoagulants and agonists on proliferation of adipose derived stromal cells (ADSCs) in auto-platelet rich plasma(PRP) gel stent. Methods ADSC, implanted into PRP gel stent using different anticoagulants (heparin, EDTA) and agonists(thrombin, collagen-1), were divided into EDTA and thrombin group, EDTA and collagen-1 group, thrombin and collagen-1 group, heparin and thrombin group, and control group. ADSC were counted everyday for 7 days and cell growth curve was plotted. Survival rate and proliferation activity of ADSC were analyzed by methyl thiazolyl tetrazolium (MTT) assay on day 2-4 and 6. Growth factor TGF- β 1 and collagen -II were detected by ELISA. Expression level of sox-9 in ADSC was measured by RT-PCR. Results The proliferation activity of ADSC was significantly higher in EDTA and thrombin group, EDTA and collagen-1 group, thrombin and collagen-1 group, heparin and thrombin group, especially in EDTA and thrombin group, than in control group(P<0.05). Auto-platelet rich plasma gel could significantly promote the release of TGF- β 1, synthesis of collagen-II , and expression of sox-9, especially in EDTA and thrombin group(P<0.05). Conclusion The effect of auto-platelet rich plasma gel is the best on proliferation of ADSC in EDTA and thrombin group.关键词
椎间盘退行性变/富血小板血浆/脂肪干细胞/组织工程/抗凝剂/激活剂Key words
disc degenerative/ platelet-rich plasma/ adipose derived stem cells/ tissue engineering/ anticoagulant/ activator分类
医药卫生引用本文复制引用
张宁,李放,罗涛..不同抗凝剂和激活剂组合在自体富血小板血浆凝胶支架促进脂肪干细胞增殖的影响[J].军医进修学院学报,2012,33(6):659-661,671,4.
