军医进修学院学报2012，Vol.33Issue(6)：662-664,3.
比较卡铂对乳腺癌细胞及血小板生成素作用下巨核细胞的影响
Effect of carboplatin on breast cancer cells and thrombopoietin on megalokaryocytes
李自健 1杨俊兰 2焦顺昌 2张帆 2谢文秀 3游俊浩2
作者信息
- 1. 解放军第161医院,肿瘤内科,湖北武汉,430012
- 2. 解放军总医院,肿瘤内科,北京,100853
- 3. 海军总医院特需医疗部,北京,100037
- 折叠
摘要
Abstract
Objective To study the effect of carboplatin on breast cancer cells and thrombopoietin(TPO) on proliferation of megalokaryocytes. Methods Effect of different TPO concentrations on megakaryocyte cell line, MO7e cells was detected by MTT assay 24, 48, and 72h after MO7e cells were exposed to TPO. A proliferation curve of MO7e cells was plotted. Twenty-four and forty-eight hour inhibition rate of different carboplatin concentrations on breast cancer MDA-MB-453 cells and TPO on MO7e cells were calculated to measure and compare their half inhibition concentration(IC50). Results TPO increased the proliferation of MO7e cells in a conceetration-dependent manner and reached its plateau when at 256ng/ml. The IC50 of MO7e cells was 10-fold higher than that of breast cancer cells. Conclusion TPO can promote the proliferation of megakaryocytes. The inhibitory effect of carboplatin on breast cancer cells is significantly higher than that of TPO on megakaryocytes.关键词
血小板生成素/MO7e细胞/卡铂Key words
thrombopoietin/ MO7e cell/ carboplatin分类
医药卫生引用本文复制引用
李自健,杨俊兰,焦顺昌,张帆,谢文秀,游俊浩..比较卡铂对乳腺癌细胞及血小板生成素作用下巨核细胞的影响[J].军医进修学院学报,2012,33(6):662-664,3.
