昆虫学报2012，Vol.55Issue(3)：276-283,8.
扶桑绵粉蚧组织蛋白酶B基因的克隆、原核表达和不同发育阶段表达分析
Molecular cloning, prokaryotic expression and expression at different developmental stages of cathepsin B gene in mealybug Phenacoccus solenopsis Tinsley ( Hemiptera: Pseudococcidae)
摘要
Abstract
Cathepsin B plays an important role in insect metabolism. In this study, cathepsin B gene was cloned from mealybug Phenacoccus solenopsis and named as PsCb ( GenBank accession no. JQ727999 ). This cDNA contains an open reading frame (ORF) which is 927 bp in length encoding 308 amino acids. Homology analysis shows that the N-terminal sequence of PsCB has larger variation, while the C-terminal sequence is conserved. Phylogenetic tree shows that PsCB is separated with other branches. Prokaryotic protein expression test showed the expressed product has the MW of 35 kDa, nearly equal to the predicted. Real-time PCR analysis revealed that PsCb mRNA was expressed in various developmental stages of P. Solenopsis. The expression level was relatively lower at the egg stage, reached a peak at the 2nd instar nymph and then declined. Our study provides a theoretical basis for further study of the gene function, the development of cathepsin inhibitors as ovicides and inhibitors of embryonic development of P. Solenopsis.关键词
扶桑绵粉蚧/组织蛋白酶B/基因克隆/原核表达/基因表达谱Key words
Phenacoccus solenopsis/ cathepsin B/ gene cloning/ prokaryotic expression/ gene expression profile分类
生物科学引用本文复制引用
罗梅,董章勇,宾淑英,廖泓之,林进添..扶桑绵粉蚧组织蛋白酶B基因的克隆、原核表达和不同发育阶段表达分析[J].昆虫学报,2012,55(3):276-283,8.基金项目
农业部公益性行业科研专项(201103026) （201103026）
仲恺农业工程学院科研基金(G3100004) （G3100004）
