昆虫学报2012，Vol.55Issue(3)：284-290,7.
中华蜜蜂DNA甲基化转移酶Dnmt3基因克隆及表达谱分析
Cloning and expression profiling of the DNA methyltransferase Dnmt3 gene in the Chinese honeybee, Apis cerana cerana (Hymenoptera:Apidae )
摘要
Abstract
To explore the pattern of methylation in the Chinese honeybee, Apis cerana cerana, the DNA methyltransferase 3 ( Dnmt3 ) gene in A. Cerana cerana was cloned by using RT-PCR, and the quantitative analysis of the expression level of Dnmt3 mRNA in different developmental stages of worker (4 d-old pupa, 1, 7 and 30 d-old workers, and laying worker) and queen (4 d-old pupa, 1 d-old queen and laying queen) were conducted using real-time qPCR. The full-length cDNA of Dnmt3 gene ( GenBank accession no. JQ740768 ) is 2 277 bp, encoding 758 amino acids, and the predicted MW and pi are 88.24 kD and 7. 85, respectively. Based on the comparison of the domain and the phylogenetic tree of the amino acids of Dnmt3 in A. Cerana cerana and other species, the sequence obtained has up to 99% identity with that of A. Mellifera. The Dnmt3 transcript was clearly detected in different developmental stages of worker and queen, and it was expressed significantly higher in 30 d-old worker than in 1 d- and 7 d-old worker (P <0.05) , while no difference existed between 1 d- and 7 d-old worker ( P > 0. 05 ). The Dnmt3 transcript was expressed higher in queen pupae than in worker pupae (P < 0.05) , and was higher in 1 d-old queen than in 1 d-old worker (P <0. 05). The expression level of Dnmt3 gene between laying worker and laying queen had no significant difference. The results suggested that Dnmt3 may be involved in the division of labor in workers and ovary development in honeybees.关键词
中华蜜蜂/DNA甲基化/DNA甲基化转移酶/基因克隆/序列分析/表达谱分析Key words
Apis cerana cerana/ DNA methylation/ DNA methyltransferase/ gene cloning/ sequence analysis/ expression profiling分类
生物科学引用本文复制引用
刘亭亭,刘俊峰,王文祥,王欢,王子龙,曾志将,颜伟玉..中华蜜蜂DNA甲基化转移酶Dnmt3基因克隆及表达谱分析[J].昆虫学报,2012,55(3):284-290,7.基金项目
江西省自然科学基金项目(2010GZN0044) （2010GZN0044）
高等学校博士学科点专项科研基金项目(20103603110003) （20103603110003）
博士后科学基金资助项目(20100481191) （20100481191）
