昆虫学报2012，Vol.55Issue(3)：316-323,8.
黄绿绿僵菌Mf82菌株对不同虫态褐飞虱的毒力
Virulence of Metarhizium flavoviride 82 to different developmental stages of the brown planthopper, Nilaparvata lugens (Hemiptera:Delphacidae)
摘要
Abstract
At present there still lacks ideal commercial microbial insecticides to control the brown planthopper ( BPH) , Nilaparvata lugens (Stil) , an important pest of rice in Asia. Entomopathogenic fungi can penetrate the cuticle of insect hosts and thus have the advantage of controlling sucking pests. In this study, laboratory bioassays were carried out on 12 isolates of three entomopathogenic fungal species, Metarhizium anisopliae, Metarhizium flavoviride and Beauveria bassiana, which were originally obtained from various hosts and geographic origins, for their virulence against BPH adults. The results showed that at the concentration of 1 100 conidia/mm2, the strain Mf82 had the highest virulence to adults with the corrected mortality reaching 83.5% within 10 d after treatment and the LT50 value 4.65 d. The pathogenicity of Mf82 to adults, nymphs at different instars and eggs of BPH were further assessed at a series of conidial concentrations. This strain was most virulent to adults, moderately virulent to old nymphs, and least virulent to young nymphs. It was also found that Mf82 could infect both oviposition marks and eggs in rice seedlings, with the infection rates of 66. 7% and 51.2% , respectively, at 10 d after treatment. The younger the eggs, the higher the infection rate, with the 0. 5 d-old eggs to be the most easily vulnerable. The results suggest that Mf82 is a promising biocontrol agent for BPH.关键词
褐飞虱/金龟子绿僵菌/黄绿绿僵菌/球孢白僵菌/毒力/致病力/侵染率Key words
Nilaparvata lugens/ Metarhizium anisopliae/ Metarhizium flavoviride/ Beauveria bassiana/toxicity/ pathogenicity/ infection rate分类
生物科学引用本文复制引用
李茂业,林华峰,李世广,金立..黄绿绿僵菌Mf82菌株对不同虫态褐飞虱的毒力[J].昆虫学报,2012,55(3):316-323,8.基金项目
国家"863"计划项目(2011AA10A205) （2011AA10A205）
公益性行业(农业)科研专项(03_200803003) （农业）
