昆虫学报2012，Vol.55Issue(3)：344-356,13.
DNA条形码技术在田间常见蓟马种类识别中的应用
Application of DNA barcoding technology for species identification of common thrips ( Insecta: Thysanoptera) in China
摘要
Abstract
Thrips (Insecta; Thysanoptera) are of a species-rich order with small size, which makes them hard to be identified quickly and accurately by traditional morphological identification methods. In this study, the mitochondrial cytochrome c oxidase subunit I ( CO I ) genes of 25 thrips species common in China were amplified using DNA barcoding universal primers. The obtained partial CO I fragments (about 650 bp) were sequenced. The phylogenetic tree was established by a neighbor-joining (NJ) method. The intra- and inter-species genetic distances were calculated with MEGA version 4. 0 software using the Kimura 2-Parameter model. The results indicated that the identification results based on the phylogenetic tree obtained was consistent with those based on morphological characteristics for the 25 thrips species. In these species, the lengths of intra-species branches were much shorter than those of the inter-species branches. Each branch corresponded to one species and all bootstrap values of the original divergence among different haplotypes within one species were 100%. The genetic distance between species for these 25 species was 102. 1 times as high as the corresponding values within species (0. 2757 vs. 0.0027). There was no overlap between inter- and intra- species genetic distances. The results suggest that the DNA barcoding based on partial CO I gene can provide rapid and accurate identification of these thrips species.关键词
缨翅目/蓟马/CoⅠ基因/DNA条形码/分子鉴定/遗传距离/系统发育树Key words
Thysanoptera/ thrips/ CO I gene/ DNA barcoding/ molecular identification/ genetic distance/ phylogenetic tree分类
生物科学引用本文复制引用
乔玮娜,万方浩,张爱兵,闵亮,张桂芬..DNA条形码技术在田间常见蓟马种类识别中的应用[J].昆虫学报,2012,55(3):344-356,13.基金项目
国家重点基础研究发展规划("973"计划)项目(2009CB119200) （"973"计划）
国家公益性行业(农业)科研专项(201103026) （农业）
国家自然科学基金项目(30971967) （30971967）
