临床肝胆病杂志2012，Vol.28Issue(3)：192-195,200,5.
左归丸粗提取物对骨髓干细胞转化肝细胞的影响
The influence of Zuogui Wan Extract on bone marrow stem cell transforming liver cell
摘要
Abstract
Objective To observe the influence of Zuogui Wan extract on bone marrow stem cell transforming liver cell, and establish experimental basis to further study effective constituent of Zuogui Wan. Methods TranswellR insert was used to establish bone marrow stem cell- liver cell co -culture system. Zuogui Wan extract was prepared by water boiled - alcohol sink method, and was added to co - culture system. Bone marrow stem cell in TranswellR insert was detected after co - culture 7, 14, 21, 28 and 35 days. Glycogen was detected by PAS method, liver cell markers (AFP, Alb and CK18) were detected by immunocytochemistry method. Results Zuogui Wan extract influenced bone marrow stem cell survive when its concentration higher than 1.56mg/ml. The expression of glycogen, AFP, Alb and CK18 in bone marrow stem cell had no significant difference contrasted to control groups, when Zuogui Wan extract's concentration was 1.56mg/ml in co- culture system. Conclusion Bone marrow stem cell was sensitive to culture condition. Traditional Chinese medicine extract prepared by water boiled - alcohol sink method was unfit for experiment of bone marrow stem cell -liver cell co -culture system.关键词
左归丸/骨髓祖代细胞/肝细胞Key words
ZUOGUI PILL/myeloid progenitor cells/hepatocytes分类
医药卫生引用本文复制引用
李瀚旻,高翔,晏雪生..左归丸粗提取物对骨髓干细胞转化肝细胞的影响[J].临床肝胆病杂志,2012,28(3):192-195,200,5.基金项目
国家自然科学基金(90709041,30672590,30271562,30371787,81102531) （90709041,30672590,30271562,30371787,81102531）
