临床肝胆病杂志2012，Vol.28Issue(3)：219-222,4.
雷帕霉素诱导大鼠肝星状细胞凋亡的研究
Study on the apoptosis of rat hepatic stellate cells induced by kapamycin
翁山耕 1徐常国 1孙颖 2石铮 1林丽娟1
作者信息
- 1. 福建医科大学附属第一医院肝胆胰、腔镜微创外科福建省腹部外科研究所,福州350005
- 2. 厦门市第二医院普外科,福建厦门361021
- 折叠
摘要
Abstract
Objective To investigate the effect of Rapamycin( RAPA)on proliferation and apoptosis of rHSCs -99 in vitro. Methods The rHSCs -99 was divided into four groups - Control group ( A) , RAPA 50 nmol/L group( B), RAPA 100 nmol/L group( C) , RAPA 150 nmol/L group ( D). After 72 h of Rapamycin interference in rHSCs - 99, the MTT colorimetric assay was used to detect the proliferation of rHSCs - 99; synthesis and secretion of collagen I were detected by ELISA; Annexin - V FICT/PI double - label immuno fluorescence with flow cytometry was used to examine the apoptosis of rHSCs - 99; Wright - Giemsa staining method was used to observe the morphologic change of rHSCs -99; Immunocytochemical staining was used to observe the proteinum expression of Fas, P53 and Bcl - 2. Results After the rHSCs - 99 were interfered with RAPA, rHSCs - 99 proliferation were inhibited; the content of type I was reduced; the number of Fas, P53 positive was increased and the Bcl - 2 positive was decreased. Conclusion RAPA inhibits the proliferation of the rHSCs - 99 and reduces the content of collagen I of ones, facilitating the cell apoptosis. The mechanism which RAPA promote rHSCs - 99 apoptosis may be through opening the Fas/FasL apoptosis pathway, up -regulating Fas,P53 and inhibiting Bcl -2 expressing to come true.关键词
肝星状细胞/雷帕霉素/细胞凋亡Key words
hepatic stellate cell/ Sirolimus/ apoptosis分类
医药卫生引用本文复制引用
翁山耕,徐常国,孙颖,石铮,林丽娟..雷帕霉素诱导大鼠肝星状细胞凋亡的研究[J].临床肝胆病杂志,2012,28(3):219-222,4.
