生物质化学工程2012，Vol.46Issue(2)：25-29,5.
HPLC法分析南方红豆杉植株中紫杉醇的分布规律
Paclitaxel Distribution in Entire Plant of Taxus chinensis var. mairei by HPLC
摘要
Abstract
Accumulation distribution of paclitaxel in entire plant of Taxus chinensis var.mairei was investigated.The content of paclitaxel in organs and tissues of T.chinensis var mairei that was wild or cultivatied about 20 years old was analyzed by HPLC.The results show that the paclitaxel in the plant is downward and has rhizosphere accumulation significantly.At the organ level,the contents of paclitaxel from branch leaves to fibrous root are 0.007 61 %-0.100 54 %（dry weight,wild） and 0.015 25 %-0.061 75 %（dry weight,cultivation） respectively.In the tissue level,the contents of paclitaxel in xylem tissue from morpholo-gical upper part to root are 0.010 95 %-0.044 76 %（dry weight,wild） and 0-0.017 58 %（dry weight,cultivation）.The accumulation of paclitaxel in skin tissue increase from morphological upper part to lower part.The contents of paclitaxel in main branch bark are 0.013 32 %（dry weight,wild） and 0.006 77 %（dry weight,cultivation）,and in root bark are 0.08633%（dry weight,wild） and 0.059 74 %（dry weight,cultivation） respectively.The output of paclitaxel in wild taxus chinensis is higher than that in cultured taxus chinensis.The distribution of paclitaxel was linear at its contents 21-105 mg/L（r2=0.999）.The average recovery was 85.78%,and the RSD was 1.03%.关键词
南方红豆杉/紫杉醇/分布/HPLCKey words
Taxus chinensis var mairei/paclitaxel/distribution/HPLC分类
化学化工引用本文复制引用
张翔宇,杜亚填..HPLC法分析南方红豆杉植株中紫杉醇的分布规律[J].生物质化学工程,2012,46(2):25-29,5.基金项目
湖南省科学技术厅科技计划项目 （）
湖南省科学技术厅科研条件创新专项 （）
吉首大学校级科研计划项目资助 （）
