临床与实验病理学杂志2012，Vol.28Issue(6)：600-603,4.
Ezrin蛋白过表达对胰腺癌细胞系Panc-1生物学行为的影响
Effect of Ezrin overexpression on pancreatic cancer cell line Panc-1
摘要
Abstract
Purpose To explore the effect of Ezrin overexpression on pancreatic cancer cell line Panc-f. Methods Plasmid-based Ezrin overexpression was stably established in pancreatic cancer cell line Panc-f. The proliferating status and cell cycle were determined by CCK-8 method and flow cytometry analysis, respectively. The cell protrusions/microvillus formation was determined by scanning e-lection microscopy. The motility and invasion of Panc-f cells were determined by transfilter assays without/with matrigel. Western blotting was used for signal transduction assay. Results Ezrin overexpression could increase cell protrusions/microvilli formation, cell motility/invasion ability of pancreatic cancer cell line Panc-f. Erkf/2 signal transduction was involved in these process. Conclusions Ezrin might have functional roles in modulating morphology, motility and invasion of pancreatic cancer cell line Panc-f, in which Erkf/2 may play an important role.关键词
胰腺肿瘤/Ezrin/微绒毛/运动/侵袭/过表达Key words
pancreatic neoplasms/ Ezrin/ microvilli/ motility/ invasion/ overexpression分类
医药卫生引用本文复制引用
孟云霄,于双妮,卢朝辉,陈杰..Ezrin蛋白过表达对胰腺癌细胞系Panc-1生物学行为的影响[J].临床与实验病理学杂志,2012,28(6):600-603,4.基金项目
国家自然科学基金资助(30471970)、国家科技支撑计划课题(2006BAI02A14)、卫生行业科研专项课题(200802011) （30471970）
