临床与实验病理学杂志2012，Vol.28Issue(6)：627-630,4.
肺腺癌和正常肺组织中抗p21ras单克隆抗体KGH-R1、KGH-R2、KGH-R3的免疫反应性比较
Immunoreactivity of anti-ras p21 monoclonal antibodies in lung adenocarcinomas and normal lung tissues
摘要
Abstract
Purpose To investigate the immunoreactivity of anti-ras p21 monoclonal antibodies KGH-R1, KGH-R2, KGH-R3 in lung adenocarcinomas and normal lung tissues. Methods Using anti-ras p21 monoclonal antibody KGH-R1, KGH-R2, KGH-R3 prepared in our laboratory, immunohistochemical SP technique was performed in 30 cases of lung adenocarcinoma and 30 cases of normal lung tissue. The immunoreactivity differences were compared between the two groups through evaluating the percentage of positive cells and histological score ( HSCORE ). Results Immunostaining of monoclonal antibodies KGH-R1, KGH-R2, KGH-R3 was found in 83.33% (25/30), 93.33% (28/30), and 63. 33% ( 19/30 ) of lung adenocarcinomas, repectively, with 100% positivity of carcinoma cells in positive cases and average HSCORES of 127. 50, 280. 25, and 158. 50. Immunostaining of the antibodies was also detected in 26. 67% ( 8/30 ), 16. 67% ( 5/30 ), and 33. 33% ( 10/30 ) of normal lung tissue with an average positive cells of 7. 75% , 7. 50% , and 8. 05% and average HSCORE of 7. 75 , 7. 50 and 8. 05 , respectively. There was no statistically significant among the average percentage of positive cells with different clones of the antibodies ( P >0. 05 ). As to HSCORE scores, KGH-R1 had a higher immunoreactivity than KGH-R2 and KGH-R3 ( P <0. 05 ). Conclusion It is suggested in the study that KGH-R1, KGH-R2, and KGH-R3 have a stronger immunoreactivity in lung adenocarcinomas than in normal lung tissues, in which KGH-R2 have the strongest immunoreactivity, suggesting that it may be a therapeutic antibody in the treatment of lung adenocarcinomas in the future.关键词
肺肿瘤/腺癌/p21ras/单克隆抗体/KGH-R1/KGH-R2/KGH-R3/免疫反应性Key words
lung neoplasms/ adenocarcinoma/ normal lung tissue/ p21ras/ monoclonal antibody/ KGH-R1/ KGH-R2/ KGH-R3 / immunoreactivity分类
医药卫生引用本文复制引用
高静,张代军,宋蜀伶,甄时建,陈玥 ,王丽,杨举伦..肺腺癌和正常肺组织中抗p21ras单克隆抗体KGH-R1、KGH-R2、KGH-R3的免疫反应性比较[J].临床与实验病理学杂志,2012,28(6):627-630,4.基金项目
国家自然科学基金(30872994) （30872994）
