临床与实验病理学杂志2012，Vol.28Issue(6)：631-635,5.
高表达p27基因对子宫颈癌HeLa细胞增殖的影响
Effect of human p27 over-expression on Hela cell proliferation
摘要
Abstract
Purpose To shirty the effect of p27 on the growth of HeLa cells by constructing a p27 overexpression cell line. Methods p27 cDNA was amplified by RT-PCR and then cloned into pcDNA3. F + vector. The resulted plasmid pcDNA3. L( + )-p27 was trans-fected into HeLa cells by lipofectinamin reagent. RT-PCR and Western blot were used to select single clones with p27 oerexpression. Effects of p27 on cell growth and cell cycle were detected by MTT method and flow cytometry assay. Results The HeLa cell lines with p27 overexpression were obtained successfully. Overexpression of p27 inhibited cell growth and caused G,/S arrest with increased G, population ( from 44. 4% to 59% ). Conclusion Overexpression of p27 gene can inhibit HeLa cell growth by interfering the cell cycles, suggesting that p27 may be a good candidate for gene therapy of carcinoma in cervix.关键词
子宫颈肿瘤/HeLa细胞/细胞周期/p27Key words
cervix neoplasms/ HeLa cell/ cell cycle/ p27分类
生物科学引用本文复制引用
尤程程,王艳林,尤昌昌,黄益玲,任东明,黄利鸣..高表达p27基因对子宫颈癌HeLa细胞增殖的影响[J].临床与实验病理学杂志,2012,28(6):631-635,5.基金项目
国家自然科学基金(30873282)、湖北省卫生厅科研项目(NX2011-9) （30873282）
