林业科学2012，Vol.48Issue(2)：169-174,6.
瑞香狼毒根的超临界萃取物抑菌活性和成分分析
Antifungal Activity of Extracts Obtained with Supercritical Carbon Dioxide Extraction from Roots of Stellera chamaejasme and Analysis of Their Constituents Using GC-MS
摘要
Abstract
In this work the supercritical fluid extraction with carbon dioxide was applied to extract roots of Stellera chamaejasme. The extracts were evaluated through the antifungal activity against Monilinia fructicola. Furthermore, the active compounds from the extracts were separated by macroporous resin and analyzed using gas chromatography-mass spectrometry ( GC-MS). The results showed that the extracts had strong antifungal activity against M. Fructicola with inhibition ratio of 88.71% at 2 000 μg . mL-1, minimum inhibitory concentration ( MIC) of 250 μg . mL-1 , and minimum fungicidal concentration ( MFC) of 2 000 μg. mL-1 . After separated with macroporous resin chromatography, fourteen fractions were collected from the extracts. Fraction 4, 7, and 12 in a concentration of 2 000 μg. mL-1 presented highly antifungal activity against M. Fructicola, with inhibition ratio of 83. 97% , 80. 23% , and 75. 69% , respectively. The GC-MS analysis revealed that the main active compounds from fraction 4, 7, and 12 included hexanedioic acid;bis(2-ethylhexyl) ester; β-sitosterol; 7-methyl-Z-tetradecen-l-ol acetate9 ; 9-hexadecenoic acid, hexadecyl ester, ( Z ) ; 1 , 2-benzenedicarboxylic acid, diisooctyl ester; (3β, 24Z ) , stigmasta-5 , 24 (28 )-dien-3-ol; stigmastan-3 , 5-diene, and squalene.关键词
瑞香狼毒/超临界萃取/抑菌活性/桃褐腐病菌Key words
Stellera chamaejasme/SC-CO2 extraction/antifungal activity/Monilinia fructicola分类
农业科技引用本文复制引用
白雪娜,谷继成,成军,师光禄,王有年..瑞香狼毒根的超临界萃取物抑菌活性和成分分析[J].林业科学,2012,48(2):169-174,6.基金项目
国家自然科学基金项目(30872029) （30872029）
北京市自然科学基金重点项目(6071001) （6071001）
北京市自然科学基金项目(6092007) （6092007）
北京市教委重点项目(KZ201010020016) （KZ201010020016）
北京市教委科研基地-科技创新平台建设项目 （）
北京市教委科技成果转化与产业化项目(PXM2011-014207-000026) （PXM2011-014207-000026）
北京市属高校人才强教深化计划资助项目(PHR20090516,PHR200906134) （PHR20090516,PHR200906134）
北京市科委重点项目(Z11100066111009). （Z11100066111009）
