林业科学2012，Vol.48Issue(5)：150-153,4.
油松种子萌发初始阶段的呼吸代谢
Respiratory Metabolism of Pinus tabulaeformis Seeds at the Initial Germinating Stages
摘要
Abstract
The seeds of Pinus tahidiformis were cultivated at (25 ± 1 ) ℃ in an incubator and the respiratory rate, activities of PGI, MDH and G-6-PDH and 6-PGDH of the seeds were measured to investigate changes in respiratory intensity and pathways during the initial germinating stages. The results showed that changes in the respiratory rate could be divided into three stages;rapid increase,stagnation and rapid increase again. The rapid increase stage occurred at the first 27 h,and the respiratory rate rose from 0.5 μmol·g-1 min to 12.79μmol·g-1 min . Within the following 18 h, respiratory rate was basically stable ranged from 12.79 to 13.55 μnol·g-1 min-1 . After 45 h incubation, the seed respiratory rate rapidly increased again. When the radicle emerged, the second peak value of respiration was 20.23 μmol og-1min-1. Activities of all respiratory enzymes increased during the initial germinating stages. The PGI activity increased rapidly and reached to the maximal value of 52. 25 OD·g-1 DW in 27 h, and then decreased rapidly. MDH activity did not change obviously in the first 36 h, and afterwards increased to a peak value of 30. 84 U·g-1min-1 in 72 h. The G-6-PDH and 6-PGDH activity increased slowly from 21. 6 U· g-1 min-1 to 29. 04 U ·g-1min-1 in the first 36 h, thereafter, the activity increased rapidly and reached to a peak value of 57. 9 U o g min" with radicle emergence. In conclusion, respiratory metabolism pathways at the early stages of incubation were mainly EMP and PPP, especially EMP pathway. Whereas,during the late stages,EMP, TCA and PPP pathways coexisted,and PPP pathway was predominant.关键词
油松/种子萌发/呼吸代谢/酶活性Key words
Pinus tabulaeformis/ seed germinating/ respiratory metabolism/ enzyme activity分类
农业科技引用本文复制引用
陈丽培,王国霞,沈永宝..油松种子萌发初始阶段的呼吸代谢[J].林业科学,2012,48(5):150-153,4.基金项目
教育部科技基础条件平台项目"林木和花卉种质资源标准化整理、整合与共享"(505002). （505002）
