棉花学报2012，Vol.24Issue(2)：120-126,7.
32个棉花主栽品种DNA指纹图谱构建及遗传多样性分析
Construction of DNA Fingerprinting and Analysis of Genetic Diversity with SSR Markers for 32 Leading Cotton Cultivars
摘要
Abstract
Thirty-two leading cotton cultivars were used as materials in the construction of DNA fingerprinting and analysis of genetic diversity with simple sequence repeat (SSR) markers. Forty primer pairs with high polymorphisms, good repeatability, and even distribution on the 26 chromosomes were screened out from 95 candidates. A total of 161 polymorphic genotypes were detected among the 32 cultivars using the 40 SSR primer pairs, with an average of 4.025. The polymorphism information content ranged from 0.2989 to 0.7585, with an average of 0.5407 per primer. Eleven primer pairs produced specific genotype bands on 13 cultivars. These 32 cotton cultivars could be absolutely identified by just 5 primer pairs. NTSYSpc v2.10 software was used to analyze genetic diversity of the cultivars. The results indicated that the cultivars from the Yangtze River valley had the highest polymorphic level, followed by those from the Yellow River valley, with the level of cultivars from the Xinjiang region being the lowest. The heritability of conventional cultivars was narrower than the hybrids.关键词
棉花/SSR/DNA/指纹图谱/遗传多样性Key words
cotton/ SSR/ DNA fingerprinting/ genetic diversity分类
农业科技引用本文复制引用
张玉翠,杨伟华,匡猛,许红霞,王延琴,周大云,冯新爱,苏畅..32个棉花主栽品种DNA指纹图谱构建及遗传多样性分析[J].棉花学报,2012,24(2):120-126,7.基金项目
中央级公益性科研院所基本科研业务费专项重点项目(SJA0904) （SJA0904）
