棉花学报2012，Vol.24Issue(3)：207-214,8.
棉花杂交种SSR核心引物的筛选与评价
Screening and Evaluation of SSR Core Primers for Indentification of Cotton Hybrids
摘要
Abstract
In order to find a set of SSR core primers with distinct polymorphic bands, good repeatability and high resolution for i-dentification of genuineness and purity of hybrid cotton varieties,we screened 2300 SSR primers from the Cotton Microsatellite Database (http://www.cottonssr.org) used on 79 cotton hybrid varieties. After two rounds of selection, 56 SSR primers were obtained, which generated 95 polymorphic loci with 1-3 loci per primer in 37 conventional hybrids; and a total of 161 genotypes were detected with 2-3 alleles per locus. Ten core SSR primers were randomly picked out, and they could clearly separated the 37 conventional hybrids by combinations. Further, 37 conventional hybrids were clustered based on UPGMA, when taking the similarity coefficient as 0.54, 20 conventional hybrids from the Yangtze River were divided into one subgroup and the other 17 ones from the Yellow River Valley into another. The results reflected the difference among different ecological regions due to the different breeding goals. The results showed this set of core primers is typical, practical, effective well to distinguish cotton hybrids.关键词
棉花/杂交种/SSR标记/核心引物Key words
cotton/ hybrid/ SSR marker/ core primer分类
农业科技引用本文复制引用
白静,聂以春,林忠旭,郭小平,张献龙,王斌,刘传祥..棉花杂交种SSR核心引物的筛选与评价[J].棉花学报,2012,24(3):207-214,8.基金项目
国家科技部转基因专项(2009ZX08005-014-B) （2009ZX08005-014-B）
国家科技支撑计划(2008BAD93B07-1) （2008BAD93B07-1）
2010年国家科技部农业科技成果转化(2010GB23600653) （2010GB23600653）
