棉花学报2012，Vol.24Issue(3)：222-228,7.
PEG介导的棉花枯萎病菌原生质体转化体系的建立
Protoplast Transformation of Fusarium oxysporum f.sp.vasinfectum Mediated by Polyethylene Glyol
摘要
Abstract
The cell walls of young hypha of Fnsarium oxysporum f.sp. vasinfectum (FOV) FOV1 and FOV4 strains were lyzed with enzymes to generate protoplasts, which were used to insert the exogenous DNA randomly under PEG8000 mediated transformation. Twenty seven and thirty nine transformants of FOV1 and FOV4 that showed hygromycin resistance and fluorescence signal were obtained, respectively. The transformation frequency of FOV1 was 1350 transformants per mg DNA and that of FOV4 was 1950 transformants per mg DNA. Three representative transformants of FOV1 and FOV4 were tested for their sporu-lation, fluorescence stability, and pathogenicity during five generations of sub-culture, and none of the transformants showed significant changes over this time. Therefore, the protoplast transformation is a stable and efficient genetic transformation method for FOV.关键词
GFP/尖孢镰刀菌/原生质体/转化/荧光信号检测Key words
GFP/ Fusarium oxysporum/ protoplast/ transformation/ fluorescence signal detection分类
农业科技引用本文复制引用
徐齐君,胡小平,陈婷,赵俊兴..PEG介导的棉花枯萎病菌原生质体转化体系的建立[J].棉花学报,2012,24(3):222-228,7.基金项目
陕西省自然科学基金(No.2010JM3018) （No.2010JM3018）
高等学校学科创新引智计划项目(B07049) （B07049）
教育部新世纪优秀人才支持计划项目(NCET-07-0698) （NCET-07-0698）
中央高校基本科研业务费专项资金(QN2009034) （QN2009034）
