可再生能源2012，Vol.30Issue(4)：80-84,5.
氨基硅烷化磁性纳米复合微球固定化纤维素酶条件优化研究
Studies on optimization of cellulase immobilization with amino-silane modified magnetic nanoparticles
摘要
Abstract
Cellulase was immobilized on the amino-silane modified magnetic nanoparticles by using glutaradehyde as the cross-linker. Firstly, the immobilization conditions were optimized through single-factor test, such as, temperature, time, pH, glutaraldehyde concentration and cellulase concentration. 20 experiments were performed according to central composite design (CCD) of three factors (temperature, pH value and glutaraldehyde concentration). Response surface model (RSM) was obtained by quadratic regression analysis of experimental data of CCD. The results showed that the optimum condition of cellulase immobilization was 21.1 ℃, pH 4.2 and 0.076 (v/v) cellulose concentration. Under optimum condition, the actual relative activity of the immobilized cellulase activity was increased,which could accord well with the predicted value.关键词
氨基硅烷化/磁性纳米Fe3O4/纤维素酶/固定化/响应面Key words
amino-silane/ Fe3O4 magnetic nanoparticles/ cellulose/ response surface method分类
能源科技引用本文复制引用
张猛,许敬亮,张宇,刘云,梁翠谊,冯胜,袁振宏..氨基硅烷化磁性纳米复合微球固定化纤维素酶条件优化研究[J].可再生能源,2012,30(4):80-84,5.基金项目
国家科技支撑计划(2011BAD22B01) （2011BAD22B01）
国家高技术研究发展计划("863"计划)(2007AA052406) （"863"计划）
中科阮知识创新工程重要方向项目(KSCX2-YW-G-075-09,KGCX2-YW-335和KSCX-YW-11-A3) （KSCX2-YW-G-075-09,KGCX2-YW-335和KSCX-YW-11-A3）
中国科学院可再生能源与天然气水合物重点实验室开放基金(0907K6). （0907K6）
