南京工业大学学报（自然科学版）2012，Vol.34Issue(2)：95-99,5.DOI:10.3969/j.issn.1671-7627.2012.02.020
提高微拟球藻油脂产量的培养工艺优化
Optimization of culture parameters to increase lipid yield from Nannochloropsis oculata
摘要
Abstract
To increase lipid yield from Nannochloropsis oculata,five culture conditions, including NaNO3 concentration, NaH2 PO4 concentration, temperature, CO2 concentration, and illumination were optimized by the method of quadratic orthogonal rotation combination experiment design. The predicted theoretical maximum lipid yield was 0.72 g/L. The optimum culture conditions of NaN03 concentration, NaH2 PO4 concentration, temperature, C02 concentration and illumination were 0. 38 g/L, 0. 35 g/L, 27. 1 °C , 6. 67% , and 5 454 lx, respectively. For the verification test,a lipid yield of 0. 70 g/L was achieved under the optimum conditions. It was increased by two fold more than that under the original conditions. Nannochloropsis oculata were dominated by C16 and C18 fatty acids.关键词
微拟球藻/培养工艺/生物柴油/二次正交旋转组合设计Key words
Nannochloropsis oculata/culture conditions/biodiesel/quadratic orthogonal rotation combination experiment design分类
生物科学引用本文复制引用
郑洪立,高振,尹继龙,黄和,纪晓俊,孙洪磊..提高微拟球藻油脂产量的培养工艺优化[J].南京工业大学学报（自然科学版）,2012,34(2):95-99,5.基金项目
国家自然科学基金重点资助项目(20936002) （20936002）
国家重点基础研究发展计划(973计划)资助项目(2011 CB200904) （973计划）
