南京农业大学学报2012，Vol.35Issue(2)：21-26,6.
不结球白菜小孢子胚胎发生过程及发育途径研究
Studies on microspore-derived embryogenesis and developmental pathway in non-heading Chinese cabbage
摘要
Abstract
The microspore embryogenesis of non-heading Chinese cabbage ( Brassica campestris ssp. Chinensis Makino) was studied using fluorescence staining technique with FDA (fluorescein diacetate). The late uninucleate stage microspores had the highest viability and exhibited the densest dyeing. The development of most microspore-derived embryos was similar to that of the zygotic embryos, but they grew faster than zygotic embryos. Globular embryos were grown into after 6-9 days of microspore culture,and coty-ledonary embryos after 13 days. We found three division patterns of microspores in sporophytic pathway. There were two patterns in the first mitosis of microspore:one was asymmetrical division,and the other was symmetrical division. Two identical or similarly sized cells were formed from the symmetrical division,and divided repeatedly to form an embryo( named B pathway). After the first asymmetrical division, both the vegetative and generative cells could be induced to generate embryos ( named E pathway) , or only the vegetative cells could be induced,but not the generative cells(named A pathway).关键词
不结球白菜/小孢子/胚胎发生/发育途径Key words
Brassica campestris ssp. Chinensis Makino/microspore/embryogenesis developmental pathway分类
农业科技引用本文复制引用
崔群香,王倩,李英,侯喜林..不结球白菜小孢子胚胎发生过程及发育途径研究[J].南京农业大学学报,2012,35(2):21-26,6.基金项目
江苏省高校青蓝工程优秀青年骨干教师项目(苏教师(2008) 30号) （苏教师(2008）
江苏省农业科技创新资金项目(cx(09)604) （cx(09）
