南京农业大学学报2012，Vol.35Issue(2)：45-50,6.
南京及周边地区螺原体菌株的系统发育及遗传多样性分析
Phylogenetic analysis and genetic diversity of spiroplasma isolates from Nanjing and its surrounding areas
摘要
Abstract
Polygenetic relationship and genetic diversity of 20 spiroplasma isolates, which were isolated from insects and flowers in Nanjing and its surrounding areas,were analyzed by phylogenetic analysis and random amplified polymorphic DNA( RAPD) markers. The results showed that most isolates had a close relationship with Spiroplasma melliferum, and were located in the Citri-Chrysopicola-Mirum clade of the phylogenetic tree generated by using the 16S rDNA sequence. Both isolate ZHUP0901 and MP0905 belonged to the Apis clade but were divided into two different sub branches. 34 RAPD random primers produced a total of 664 alleles.of which 644 were polymorphic with a polymorphism percentage of 96.99%. The UPGMA dendrogram suggested that all spiroplasma isolates were divided into 5 groups when similarity coefficient was 0. 6. Eighteen spiroplasma isolates clustered with Spiroplasma melliferum were divided into 3 groups according to their different hosts. ZHUFD901 and MF0905 each formed an independent group. The spiroplasmas isolated from different hosts (plant flowers, bee and other insects) during the same period had a close relationship with each other,indicating that spiroplasmas were transmitted by horizontal transmission in nature.关键词
螺原体/系统发育分析/遗传多样性/RAPDKey words
Spiroplasma sp./phylogenetic analysis/genetic diversity/RAPD分类
生物科学引用本文复制引用
李霞,于汉寿,回丽静,刘淑园,阮康勤,陈永萱..南京及周边地区螺原体菌株的系统发育及遗传多样性分析[J].南京农业大学学报,2012,35(2):45-50,6.基金项目
国家自然科学基金项目(30870002) （30870002）
