南京医科大学学报（自然科学版）2012，Vol.32Issue(3)：306-309,373,5.
耐吉西他滨胰腺癌细胞株SW1990/GZ上皮间质转化现象的研究
Investigation of epithelial-mesenchymal transition in gemcitabine-resistant cancer cell line SW1990/GZ
摘要
Abstract
Objective:To explore epithelial-mesenchymal transition (EMT) in gemcitabine-resistant cancer cell line SW1990/GZ. Methods; Morphological characteristics of SW1990 cells and SW1990/GZ cells were observed by inverted microscope. Cell cycle distribution was analyzed by flow cytometry. The expressions of EMT-associated markers were determined by Western blot. Invasion and migration of cells were detected by transwell. The proportion of cancer stem cells was analyzed by flow cytometry after labeled the surface specific antigen. Results; SW1990/GZ cells exhibited mesenchymal morphology,and no significant G1 accumulation pheno type in SW1990/GZ cells compared with SW1990 cells. SW1990/GZ cells reduced expression of the epithelial marker E-cadherin but increased expression of the mesenchymal marker vimentin. SW1990/GZ cells have greater invasion and migration activity (P < 0.01). Compared with SW1990 cells,the number of CD44+CD24+ cells and CD 133+ cells increased by 2 times and 4 times in SW1990/GZ cells(P < 0.01). Conclusion;SW1990/GZ cells have undergone an EMT,and are enriched with cancer stem cells.关键词
胰腺癌/上皮间质转化/肿瘤干细胞/侵袭/迁移Key words
pancreatic cancer/epithelial-mesenchymal transition/cancer stem cell/invasion/migration分类
医药卫生引用本文复制引用
王辉,张烨,吴峻立,徐泽宽,苗毅..耐吉西他滨胰腺癌细胞株SW1990/GZ上皮间质转化现象的研究[J].南京医科大学学报（自然科学版）,2012,32(3):306-309,373,5.基金项目
国家自然科学基金资助(30972912) （30972912）
