慢病毒介导的miR-27a下调对U87胶质瘤细胞的影响OA北大核心CSCDCSTPCD
Effect of miR-27a knockdown mediated by lentiviral vector on U87 glioma cell
目的:探讨下调miR-27a对U87胶质瘤细胞的影响.方法:分别用慢病毒干扰质粒miRZip-27a anti-miR-27a microRNA construct或慢病毒空质粒pGreenPuro Scramble Hairpin Control-Construct与慢病毒包装质粒混合物共转染293T细胞,获得慢病毒颗粒命名为Lt-I和Lt-NC.然后分别用慢病毒Lt-I和Lt-NC感染U87胶质瘤细胞,通过流式细胞仪筛选出稳定感染的U87胶质…查看全部>>
Objective: To explore the effect of miR-27a knockdown on U87 glioma cells in vitro. Methods; The lentiviral vector miRZip-27a anti-miR-27a microRNA construct,empty lentiviral vector pGreenPuro Scramble Hairpin Control-Construct,and lentiviral packaging plasmids in supernatant were collected and transfected into 293T cells,the packaged lentivirus were named as Lt-I and Lt-NC. Then the packaged lentivirus Lt-I and Lt-NC were used to infect U87 glioma cells.the…查看全部>>
包庆泉;冯素银;邵君飞
南京医科大学附属无锡市人民医院神经外科,江苏无锡214023苏州大学附属第四医院神经外科,江苏无锡214062南京医科大学附属无锡市人民医院神经外科,江苏无锡214023
医药卫生
神经胶质瘤miR-27a慢病毒
gliomamiR-27alentivirus
《南京医科大学学报（自然科学版）》 2012 (3)
320-324,5
江苏省自然科学基金资助(BK2009078)
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