南京医科大学学报（自然科学版）2012，Vol.32Issue(3)：325-328,4.
S100A16转基因小鼠的构建与鉴定
Establishment and identification of S100A16 transgenic mice
摘要
Abstract
Objective:To establish a transgenic mouse model systemic expression S100A16,which can be used for the study of its biologic function. Methods: The plasmid was generated by inserting the murine S100A16 cDNA into a vector with CMV promoter. The transgenic mice were produced by the method of microinjection. The genotype of transgenic lines was identified by PCR and the expression level of the gene was determined by QPCR. Results; S100A16 cDNA transgenic vector was successfully constructed and S100A16 transgenic mice were created. Two high expression lines were determined by QPCR. Conclusion; The transgenic mouse systemic expressing S100A16 was successfully established,and the S100A16 gene was highly expressed in many tissues,such as adipose tissue,liver,muscles,and lung. The S100A16 mouse could be a useful model for the researches of its function,especially in obesity and insulin resistance.关键词
S100A16基因/转基因小鼠/肥胖/胰岛素抵抗Key words
S100A16 gene/transgenic mice/obesity/insulin resistance分类
生物科学引用本文复制引用
杜新丽,薛一,张媛媛,张日华,刘云..S100A16转基因小鼠的构建与鉴定[J].南京医科大学学报（自然科学版）,2012,32(3):325-328,4.基金项目
国家自然科学基金(81070684),江苏省科技支撑计划(BE2011802),江苏省“六大人才高峰”项目,南京医科大学第一附属医院创新团队工程资助 （81070684）
