南京医科大学学报（自然科学版）2012，Vol.32Issue(3)：349-353,5.
高糖经内质网应激途径诱导胰岛内皮细胞凋亡
Hyperglycemia induced islet endothelial cell line MS-1 apoptosis through endoplasmic reticulum stress pathway
摘要
Abstract
Objective:To investigate the effect of hyperglycemia on the apoptosis and proliferation of islet endothelial cell line MS-1. Methods; MS-1 cells were treated with different concentrations of glucose (5.6,25.0 and 33.6 mmol/L) for different time (12 or 24 h). The apoptotic cells were detected by flow cytometry.and the cell proliferation was tested using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay. The expressions of GRP78,CHOP,caspase-3,caspase-12 Mrna were examined by reverse transcription and polymerase chain reaction(RT-PCR),and the expressions of GRP78,CHOP protein were examined by Western blot. Results; After treated with glucose for 12 h or 24 h,the apoptotic rates were obviously higher in 25.0 and 33.6 mmol/L group than in 5.6 mmol/L group(P < 0.05),while the cell proliferation was significantly lower in 25.0 and 33.6 mmol/L glucose treated group than that in 5.6 mmol/L glucose treated group (P < 0.05). The Mrna levels of CHOP,caspase-3,caspase-12 and the protein level of CHOP increased in a dose-dependent manner after treated with glucose (P < 0.05); while the Mrna and protein levels of GRP78 increased after treated for 12 h,and decreased at 24 h (P < 0.05). Conclusion; Hyperglycemia significantly promotes apoptosis and decreases proliferation of MS-1 cells,the mechanism may account for endoplasmic reticulum stress.关键词
胰岛内皮细胞/高糖/凋亡/增殖/内质网应激Key words
islet endothelial cells/ hyperglycemia/ apoptosis/ proliferation/ endoplasmic reticulum stress分类
医药卫生引用本文复制引用
刘璇,徐宽枫,陈恒,许馨予,杨涛..高糖经内质网应激途径诱导胰岛内皮细胞凋亡[J].南京医科大学学报（自然科学版）,2012,32(3):349-353,5.基金项目
国家重点基础研究发展计划(973计划)(2010CB535008) （973计划）
