南京医科大学学报（自然科学版）2012，Vol.32Issue(3)：359-363,5.
背根神经节微量注射7-硝基吲唑抑制炎症性疼痛
Suppression of inflammatory pain by microinjecting 7-nitroindazole into dorsal root ganglion
摘要
Abstract
Objective:To observe the phenomenon of complete Freund's adjuvant (CFA)-induced inflammatory pain and explore the analgesic effect of 7-nitroindazole (7-NI) on dorsal root ganglion (DRG) ,and briefly discuss its analgesic mechanism. Methods: Ten microliters of CFA were injected into the plantar surface of the hind paw of the mice to produce inflammatory pain models,von Frey filaments were used to determine 50% paw withdrawal threshold(PWT) adopting by up-and-down method which can be regarded as an evaluation criteria for the pain behavior test The changes of 50% PWT were tested by microinjecting 10 μg 7-NI into DRG of the mice. Detect the expression of calcitonin gene related pepn'de(CGRP) in CFA-induced,7-NI treated and neuronal nitric oxide synthase(nNOS) knock-out models by Western blot Results: Compared with DMSO group,50% PWT significantly increased in 7-NI group during 2~12 h after CFA inducing. The expression of CGRP increased after CFA injection,this effect could be reversed by 7-NI administration or nNOS knock-out Conclusion: Microinjection of 7-NI into DRG could produce analgesia in inflammatory pain mice. Increased expression of CGRP might be the downstream mechanism resulted from NO mediated nociceptive effect in dorsal root ganglion.关键词
神经元型一氧化氮合酶/7-硝基吲唑/炎症性疼痛/降钙素基因相关肽Key words
Nnos/7-NI/inflammatory pain/CGRP分类
医药卫生引用本文复制引用
孙伟翔,徐海兵,朱东亚..背根神经节微量注射7-硝基吲唑抑制炎症性疼痛[J].南京医科大学学报（自然科学版）,2012,32(3):359-363,5.基金项目
江苏省自然科学基金重点研究专项(BK2011029) （BK2011029）
