南京医科大学学报（自然科学版）2012，Vol.32Issue(3)：395-398,4.
荧光定量PCR检测膀胱癌患者尿沉淀细胞TWIST1基因启动子甲基化状态的临床价值
Detection of the TWIST1 promoter methylation state through fluorescent quantitative PCR in urine sediments of bladder cancer
陆明 1钱麟 1潘彬 1陈建刚 1赵枰1
作者信息
- 1. 南通市第一人民医院泌尿外科,江苏南通226001
- 折叠
摘要
Abstract
Objective:To assess the TWIST1 promoter methylation state in urine sediments and evaluate their value in diagnosis of bladder cancer. Methods: Fifty patients with bladder cancer, 13 patients with non tumorous urinary disease and 7 healthy volunteers were recruited in the study. The TWIST1 promoter methylation state in urine sediments was determined by real-time fluorescent quantitative PCR. The urine exfoliative cytologic examination was also performed. Results: The positive rate of TWIST1 promoter methylation was 64.0% (32/50) in exfoliated urothelial cells of 50 patients with bladder cancer, no one showed positive TWIST1 promoter methylation in the control group. The difference in the positive rate of TWIST1 promoter methylation between two groups was significant(P < 0.01). TWIST1 promoter methylation status did not correlate with stage and grade of bladder cancer,though there was a trend that more frequent methylation was detected in higher stage bladder cancer. The sensitivity of detecting TWIST1 promoter methylation by real-time fluorescent quantitative PCR in the diagnosis of bladder cancer was 64.0%,and specificity was 100%. The positive rate of urine exfoliative cytology was 48.0%(24/50),and sensitivity and specificity were 48.0% and 100.0% respectively. Conclusion: It has high sensitivity and specificity to detect the TWIST1 promoter methylation in exfoliated urothelial cells for the diagnosis of bladder cancer. The TWIST1 promoter methylation might be a potential biomarker for the noninvasive and painless detection method in early diagnosis of bladder cancer.关键词
膀胱癌/甲基化/实时荧光定量PCRKey words
bladder carcinoma/methylation/real-time fluorescent quantitative PCR分类
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陆明,钱麟,潘彬,陈建刚,赵枰..荧光定量PCR检测膀胱癌患者尿沉淀细胞TWIST1基因启动子甲基化状态的临床价值[J].南京医科大学学报（自然科学版）,2012,32(3):395-398,4.
