农业机械学报2012，Vol.43Issue(2)：146-150,204,6.DOI:10.6041/j.issn.1000-1298.2012.02.029
条斑紫菜ACEI抑制肽的超滤分离与离子交换纯化
Ultrafiltration and Ionic Exchange Purification of ACE Inhibitory Peptides from Hydrolysate of Porphyra yezoensis Protein
摘要
Abstract
Angiotensin I-converting enzyme (ACE) inhibitory peptides from digested Porphyra yezoensis protein were purified with ultrafiltration and ionic exchange. The hydrolysate of Porphyra yezoensis protein was ultrafiltered by 10 kDa and 3 kDa membrane in sequence. It was found that the IC50 value of Porphyra yezoensis peptides decreased from original 0. 844 mg/mL to 0. 523 mg/mL. And Nakai D61 cation resin was selected according to its largest absorption in the ionic exchange static absorption test. The results showed that the second elution peak had the highest protein recovery, ACE inhibitory activity recovery and the lowest IC50 value, which were 45. 2% , 61. 5% and 0. 127 mg/mL, respectively. The sample's IC50 value decreased by 75. 7% after the ionic exchange purification.关键词
条斑紫菜/ACEI抑制肽/超滤/离子交换Key words
Porphyra yezoensis / ACE inhibitory peptides/ Ultrafiltration/ Ionic exchange分类
轻工纺织引用本文复制引用
何荣海,马海乐,余筱洁..条斑紫菜ACEI抑制肽的超滤分离与离子交换纯化[J].农业机械学报,2012,43(2):146-150,204,6.基金项目
中国博士后科学基金资助项目(20100471386)、江苏省博士后科学基金资助项目(0902029C)、浙江省自然科学基金资助项目(Y3110025)、江苏高校优势学科建设工程资助项目、南通市产学研联合创新科技计划资助项目(B2010008)和江苏大学高级人才科研启动基金资助项目(08JDG032) （20100471386）
