农业机械学报2012，Vol.43Issue(5)：122-129,8.DOI:10.6041/j.issn.1000-1298.2012.05.021
基于中药乙醇提取物的柑橘采后保鲜与抑菌技术
Fresh-keeping and Antibacterial Technology of Citrus Fruits Based on Ethanol Extracts from Chinese Herbs
摘要
Abstract
The plant resources with inhibitory effect on postharvest pathogenic fungi from citrus fruits were screened so as to provide the theoretical basis for the development of botanical fungicide. The experiments investigated inhibitory effects in vitro with 30 kinds of ethanol extracts from Chinese herbs against P. italicum, P. digit alum and Alternaria, citri by using mycelial growth rate method, and control effects in vivo and storage effects of Valencia orange with Coptis chinensis ethanol extracts. The results showed that 30 kinds of Chinese herbs ethanol extracts have different effects on mycelial growth of three citrus pathogens, and Coptis chinensis ethanol extracts exhibited the best antifungal effect which reached up to 100%. However, no difference was found between Prochloraz treatment and Coptis chinensis treatment (P> 0.05). Minimum inhibitory concentration (MIC) of Coptis chinensis ethanol extracts is 0. 025 g/mLu Control test showed that the control effect of Coptis chinensis ethanol extracts against the mixtures of three kinds of spores achieved 90. 87% after 10 d of treatment, and there was no significant difference ( P > 0. 05 ) between Coptis chinensis treatment and Prochloraz treatment. Preservation test indicated that Coptis chinensis ethanol extracts could obviously decrease the incidence of rotting rate and molding of Valencia orange fruits, increase the good fruit rate, and retard the losses of titratable acidity and vitamin C in fruits. Compared with Prochloraz treatment, there was no difference (P >0. 05). These results indicated that in vitro experiments, control effect and preservation test exhibited a good antifungal effect with Coptis chinensis ethanol extracts. Therefore, Coptis chinensis ethanol extracts may have a possibility to replace chemical fungicides and a new pesticide can be developed.关键词
柑橘/中药乙醇提取物/抑菌/贮藏Key words
Citrus/ Ethanol extracts from Chinese herb/ Antibacterium/ Preservation分类
农业科技引用本文复制引用
任艳芳,刘畅,何俊瑜,王思梦,张兰兰..基于中药乙醇提取物的柑橘采后保鲜与抑菌技术[J].农业机械学报,2012,43(5):122-129,8.基金项目
贵州省农业科技攻关资助项目(NY20083021) （NY20083021）
