农业生物技术学报2012，Vol.20Issue(1)：62-69,8.DOI:10.3969/j.issn.1674-7968.2012.01.009
马铃薯腐烂茎线虫的遗传变异及其与生态因子的相关性
Genetic Variation of Ditylenchus destructor and Its Association with Ecological Factors
摘要
Abstract
Ditylenchus destructor is a quarantine plant-parasitic nematode that distributed worldly. The genetic variation of D. Destructor may be modified with the change of ecological environment. The effect of ecological factor on the genetic structure may be induced by the genetic variation of different population of Ditylenchus destructor. The genetic diversity and genetic differentiation of D. Destructor were analyzed with inter-simple sequence repeat (ISSR) molecular marker in this study. The effect of ecological factor on genetic diversity of D. Destructor was analyzed with SPSS software. Results showed that genetic diversity was relative high in D. Destructor. At the population level, the higest genetic diversity existed in the Taihe population of Anhui Provinbe, the lowest genetic diversity existed in the Changli population of Hebei Province. At the group level, the genetic diversity of five groups was arranged as Anhui >Shandong >Jiangsu >Hebei >Beijing. Relativey low genetic differentiation and high gene flow were existed in five groups with which coefficient of genetic differentiation^,) was 0.0725 and gene flow (Nm) was 6.3933. Correlation analysis showed a positive relationship between Shannon genetic diversity and altitude, longitude, latitude and annual precipitation, while a negative relativeship existed between Shannon genetic diversity and average temperature, maximum temperature and minimum temperature. A positive relationship also existed between genetic distance and geographical distance. The results imply that D. Destructor adapts to low temperature and it will be suitable to propagate in northern China. The management of seedling and seedtuber of sweet potato (Solarium tuberosum) shall be reinforced to prevent it's distribution and spreading in northern China.关键词
马铃薯腐烂茎线虫/简单重复序列区间(ISSR)/遗传变异/生态因子/相关分析Key words
Ditylenchus destructor/Inter-simple sequence repeat(ISSR)/ genetic differentiation/ecological facto/ correlation analysis引用本文复制引用
黄文坤,何旭峰,丁中,彭德良..马铃薯腐烂茎线虫的遗传变异及其与生态因子的相关性[J].农业生物技术学报,2012,20(1):62-69,8.基金项目
国家自然科学基金(No.31071668) （No.31071668）
