农药学学报2012，Vol.14Issue(2)：143-150,8.DOI:10.3969/j.issn.1008-7303.2012.02.05
胶孢炭疽菌及两种疫霉菌对苯酰菌胺的敏感性与β-微管蛋白氨基酸突变的相关性分析
Correlation between sensitivity to zoxamide and the mutation of β-tubulin amino acid of Colletotrichum gloeosporioides and two Phytophthora spp.isolates
摘要
Abstract
The correlation between mutations of β-tubulin amino acid and the sensitivity of Colletotrichum gloeosporioides, Phytophthora capsici and P. Cactorum isolates to carbendazim, diethofencarb and zoxamide was investigated by the methods of the mycelial growth inhibition and amino acid sequence alignment. The results revealed that C. Gloeosporioides isolates exhibiting different sensitivity to zoxamide, carbendazim and diethofencarb were associated with the mutations in position 198 or 200 on β-tubulin amino acid sequence. Carbendazim-sensitive isolates of C. Gloeosporioides with glutamic acid (E) and phenylalanine (F) at 198 and 200 sites respectively in β-tubulin amino acid sequence were insensitive to diethofencarb and zoxamide. A substitution of tyrosine (Y) for glutamic acid ( F) in position 200 was detected in carbendazim-resistant but diethofencarb and zoxamide insensitive isolates. Glutamic acid (E) to alanine (A) change at position 198 was identified in carbendazim high resistant isolates that were sensitive to diethofencarb and zoxamide. All tested P. Capsici and P. Cactorum isolates were sensitive to zoxamide, but insensitive to carbendazim and diethofencarb. No amino acid sequence difference was detected among all Phytophthora isolates,but the sites from 196 to 200 in β-tubulin amino acid sequence of Phytophthora spp. Were significantly different from that of C. Gloeosporioides. These might be the reason for the sensitivity of Phytophthora spp. To zoxamide only.关键词
苯酰菌胺/疫霉菌/胶孢炭疽菌/β-微管蛋白/敏感性Key words
zoxamide/ Phytophthora/ Colletotrichum gloeosporioides /β-tubulin/ sensitivity分类
农业科技引用本文复制引用
邓维萍,朱书生,梅馨月,杨敏,陈磊,毕扬,蔡萌,王海燕,阿普前,何霞红..胶孢炭疽菌及两种疫霉菌对苯酰菌胺的敏感性与β-微管蛋白氨基酸突变的相关性分析[J].农药学学报,2012,14(2):143-150,8.基金项目
云南省科技创新强省计划(2007AB007) （2007AB007）
国家自然科学基金项目(30800731) （30800731）
高校博士点基金(200800190025). （200800190025）
