农药学学报2012，Vol.14Issue(2)：151-157,7.DOI:10.3969/j.issn.1008-7303.2012.02.06
小菜蛾中肠氨肽酶N2在昆虫细胞中的表达
Expression of aminopeptidase N (APN2) from Plutella xylostella larval midgut in Sf9 cells
摘要
Abstract
Using the insect cells-Bac-to-Bac baculovirus expression system to express APN2 (aminopeptidase N) protein, the APN2 expression system in Sf9 cells was successfully established. APN2 gene from susceptible and resistant Plutella xylostella (L. ) larval midgut were inserted into pFAST Bac HTB vector, expressed in insect cells Spodoptera frugiperda (Sf9) cells, respectively, with Bac-to-Bac baculovirus expression system. A 107 kDa protein was examined and confirmed by SDS-PAGE and Western-blotting. The results showed that P. Xylostella larval midgut APN2 gene was successfully expressed in Sf9 cells which provided a foundation for further investigation on function of APN2 and its relationship with Bt resistance.关键词
苏云金芽孢杆菌/氨肽酶N/绿色荧光蛋白/Sf9细胞系/杆状病毒表达系统Key words
Bacillus thuringiensis/aminopeptidase N/ green fluorescent protein ( GFP)/ Spodoptera frugiperda (Sf9) cells/ Bac-to-Bac baculovirus expression system分类
生物科学引用本文复制引用
王俊华,周小毛,吴青君,王少丽,谢文,陈得峰,徐宝云,张友军..小菜蛾中肠氨肽酶N2在昆虫细胞中的表达[J].农药学学报,2012,14(2):151-157,7.基金项目
国家高技术研究发展计划(863计划)(2012AA101502) （863计划）
公益性行业(农业)科研专项(201103021). （农业）
