中国海洋大学学报（自然科学版）2012，Vol.42Issue(4)：55-58,4.
禽流感病毒抗原表位分析及H5N1亚型基因工程疫苗设计
Analysis of Avian Influenza Virus Epitopes and the Design of H5N1 Virus Genetic Engineering Vaccine
刘学东 1王志亮 2包振民3
作者信息
- 1. 中国海洋大学海洋生物遗传与种质工程实验室,山东青岛266003
- 2. 青岛市市立医院呼吸科,山东青岛266003
- 3. 中国农业部动物检疫所外来病中心,山东青岛266033
- 折叠
摘要
Abstract
In this study, we choose the asian H5N1 subtype avian influenza virus, use software to analyze the gene sequences of the HA1 (Hemagglutnin, HA, hemagglutinin) and NP (Nucleocapsid protein, capsid protein), and optimize major T cell epitopes and B cell epitopes of the HA1 protein and the major CTL (Cytotoxicity T lymphocyte cytotoxic T lymphocyte) epitope of the NP protein. According to these preferred epitopes, we designed the avian influenza virus subtype H5N1 recombinant vaccine. Genetically engineered vaccine expression vector pRSET-AIV was constructed, exogenous gene can be well expressed in E, coli system. Mice immunized with expression products, serum IgA and IgG antibody levels were significantly increased, IL-2, IL-4 and IFN-y cytokines were tested in vitro spleen cells. The antigen of genetic engineering was verified. It's confirmed that the vaccine stimulates the cellulat's immunity while it activates the humoral immunity.关键词
禽流感/基因工程疫苗/抗原表位/H5N1Key words
avian influenza/ vaccine/ epitope/ H5N1分类
生物科学引用本文复制引用
刘学东,王志亮,包振民..禽流感病毒抗原表位分析及H5N1亚型基因工程疫苗设计[J].中国海洋大学学报（自然科学版）,2012,42(4):55-58,4.
