中国海洋大学学报（自然科学版）2012，Vol.42Issue(6)：77-80,4.
半滑舌鳎三倍体诱导研究
Study on Triploid Induction in the Half-Smooth Tongue Sole (Cynoglossus semilaevis)
摘要
Abstract
The second polar body extrusion was inhibited to optimize the conditions of cold shock for producing triploid half smooth tongue sole (Cynoglossus semilaevis). Triploidy was confirmed successfully by flow cytometry and chromosome observations. The highest triploidy rate of 35% was achieved by treating the fertilized eggs for 20 minutes in 4 ℃ seawater starting at 5 minutes after fertilization. Male and Female chromosome types were determined to be ZZZ and ZWW, and appeared in 1 : 1 ratio. ZZW chromosome type was not observed, which suggested that crossing over between Z and W chromosomes was inhibited in half-smooth tongue sole.关键词
半滑舌鳎/三倍体/冷休克Key words
Cynoglossus semilaevis /triploidy/cold shock分类
农业科技引用本文复制引用
刘志鹏,王旭波,翟介明,张全启..半滑舌鳎三倍体诱导研究[J].中国海洋大学学报（自然科学版）,2012,42(6):77-80,4.基金项目
山东省"泰山学者"建设工程专项资助 （）
