四川大学学报（自然科学版）2012，Vol.49Issue(2)：431-435,5.DOI:10.3969/j.issn.0490-6756.2012.02.032
农杆菌介导的棉花遗传转化过程中诸因素对出愈率的影响
Effects of some factors on callus rate in the process of cotton genetic transformation mediated by Agrobacterium tumefaciens
摘要
Abstract
Some related factors influencing explants callus rate in the process of cotton genetic transformation mediated by Agrobacterium tumefaciens were studied. The results showed that the optimal concentration of Kanamycin(Kan) was 50 mg/L or 75 mg/L,the ability of disseminating of Agrobacterium tumefaciens EHA105 was more powerful, the optimal material of explant was hypocotyl of 7-days-old seedling,the pre-incubated hypocotyl explants before ?disseminated by Agrobacterium tumefaciens were more favorable to the formation of embryogenic callus,and the optimal period of disseminating was 3 minutes.关键词
棉花/外植体/农杆菌/出愈率Key words
cotton, explants, Agrobacterium tumefaciens, callus rate分类
农业科技引用本文复制引用
孙英坤,代其林,龚元亚,田霞,谢琳,杨娟,王劲..农杆菌介导的棉花遗传转化过程中诸因素对出愈率的影响[J].四川大学学报（自然科学版）,2012,49(2):431-435,5.基金项目
转基因生物新品种培育重大专项(2009ZX08009-091B) （2009ZX08009-091B）
国家自然科学基金(30871555) （30871555）
教育部新世纪优秀人才支持计划(NCET-08-0940) （NCET-08-0940）
四川省教育厅(09ZA034) （09ZA034）
